Найти книгу: "Protein Sequencing and Identification Using Tandem Mass Spectrometry"

How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes: Peptide fragmentation and interpretation of product ion spectra Basic polyacrylamide gel electrophoresis Preparation of protein digests for sequencing experiments Mass spectrometric analysis using capillary liquid chromatography Techniques for protein identification by database searches Characterization of modified peptides using tandem mass spectrometry And much more

The RAW file format is the uncompressed data file captured by a digital camera's electronic sensor. When your camera saves an image in RAW format, settings like white balance, sharpening, contrast and saturation are not applied to the image but are saved instead in a separate header. Because RAW files remain virtually untouched by in-camera processing, they are essentially the digital equivalent to exposed but undeveloped film. This makes RAW an increasingly popular format with amateur and professional digital photographers, because it affords greater flexibility and control during the editing process-if you know how to work with RAW files. Most digital camera manufacturers supply their own software for converting RAW data, as do some third party vendors. Increasingly, however, the RAW converter of choice is a plug-in included in the latest version of Adobe Photoshop, the most popular and widely-used digital image editing tool in the world. Adobe Photoshop CS2 is emerging as the best place to edit RAW images, and the best way to master this new format is with Photoshop CS2 RAW. An important book dedicated to working with RAW in Photoshop, this comprehensive guide features a unique design that helps readers grasp the subject through visual instruction and prompts. The entire RAW process is explored, from shooting to using the Adobe plug-in converter and new Bridge navigation software. The primary focus of Photoshop RAW is, as the title suggests, Photoshop editing technique: automating RAW workflow, correcting exposures, extending exposure range, manipulating grayscale and working with the new DNG (Digital Negative) open standard that Adobe supports. Presented by photographer Mikkel Aaland, a pioneer of digital photography and author of eight books, including O"Reilly"s Photoshop for the Web and the award-winning Shooting Digital, Photoshop CS2 RAW investigates and instructs in an accessible visual style. Required reading for professionals and dedicated photo hobbyists alike.

В гостях у основателя бизнес-школы «ВВЕРХ», эксперта по созданию команд №1 в России Владимира Мариновича, Корнелий Сао – владелец мебельной компании TANDEM. В ходе беседы Владимир и его гость раскроют основные факторы создания эффективной компании: путь в бизнес; создание продукта; создание и развитие команды; книги и люди, которые мотивируют.

This book is a kind of a door, through which you will get to know the famous musicians via their relationships with the surrounding world and their colleagues. Here you will learn how the musicians spoke about one another, whom they loved and whom they did not like, with whom they were friends and with whom they competed. In addition, you will learn how their music is born and the beneficial impact it has on us.

It is very important to generate a good attitude, a good heart, as much as possible. From this, happiness in both the short term and the long term for both yourself and others will come. Dalai Lama XVI