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Designed as a research-level guide to current strategies and methods of membrane protein production on the small to intermediate scale, this practice-oriented book provides detailed, step-by-step laboratory protocols as well as an explanation of the principles behind each method, together with a discussion of its relative advantages and disadvantages. Following an introductory section on current challenges in membrane protein production, the book goes on to look at expression systems, emerging methods and approaches, and protein specific considerations. Case studies illustrate how to select or sample the optimal production system for any desired membrane protein, saving both time and money on the laboratory as well as the technical production scale. Unique in its coverage of «difficult» proteins with large membrane-embedded domains, proteins from extremophiles, peripheral membrane proteins, and protein fragments.

Widely used in civil, mechanical and automotive engineer­ing since the early 1980s, multilayer rubber bearings have been used as seismic isolation devices for buildings in highly seismic areas in many countries. Their appeal in these applications comes from their ability to provide a component with high stiffness in one direction with high flexibility in one or more orthogonal directions. This combination of vertical stiffness with horizontal flexibility, achieved by reinforcing the rubber by thin steel shims perpendicular to the vertical load, enables them to be used as seismic and vibra­tion isolators for machinery, buildings and bridges. Mechanics of Rubber Bearings for Seismic and Vibration Isolation collates the most important information on the mechanics of multilayer rubber bearings. It explores a unique and comprehensive combination of relevant topics, covering all prerequisite fundamental theory and providing a number of closed-form solutions to various boundary value problems as well as a comprehensive historical overview on the use of isolation. Many of the results presented in the book are new and are essential for a proper understanding of the behavior of these bearings and for the design and analysis of vibration or seismic isolation systems. The advantages afforded by adopting these natural rubber systems is clearly explained to designers and users of this technology, bringing into focus the design and specification of bearings for buildings, bridges and industrial structures. This comprehensive book: includes state of the art, as yet unpublished research along with all required fundamental concepts; is authored by world-leading experts with over 40 years of combined experience on seismic isolation and the behavior of multilayer rubber bearings; is accompanied by a website at www.wiley.com/go/kelly The concise approach of Mechanics of Rubber Bearings for Seismic and Vibration Isolation forms an invaluable resource for graduate students and researchers/practitioners in structural and mechanical engineering departments, in particular those working in seismic and vibration isolation.

A comprehensive overview of biodegradable polymers, covering everything from synthesis, characterization, and degradation mechanisms while also introducing useful applications, such as drug delivery systems and biomaterial-based regenerative therapies. An introductory section deals with such fundamentals as basic chemical reactions during degradation, the complexity of biological environments and experimental methods for monitoring degradation processes. The result is a reliable reference source for those wanting to learn more about this important class of polymer materials, as well as scientists in the field seeking a deeper insight.

The new edition of this popular and widely-used American history textbook has been thoroughly updated to include a wealth of new scholarship on American diplomacy in the decade leading up to Japan’s attack on Pearl Harbor. Features new material on the Washington Conference of 1921-22, early American diplomacy in the Manchurian crisis, the Panay incident, Russia’s invasion of Finland, the destroyer-bases deal, and much more Pays particular attention to Roosevelt’s policies towards Jewish refugees, the battle between domestic groups like the America First Committee and Fight for Freedom, and the Welles mission of 1940 Includes concise biographical sketches of major world leaders, including Hoover, FDR, Churchill, Hitler, Stalin, Mussolini, and Tojo Outlines and examines the debates of historians over the wisdom of U.S. policies

Written by experienced authors, this book presents numerous natural everyday products with a high range of structural diversity. Twenty natural products have been arranged in five sections, describing three alkaloids, five colored compounds, three carbohydrates and glycosides, seven terpenoids, and two aromatic compounds. Adopting a highly didactical approach, each chapter features a uniform structure: Background, in-depth information about isolation processes and structural characterization as well as a Q&A section at the end. Alongside the theoretical information many practical hints for the laboratory work are also included. A comprehensive overview of UV-, IR- and NMR-spectroscopy as well as mass-spectrometry for every exemplified compound is provided and the understanding of these methods is supported by concluding questions and exercises. Educating and entertaining, this full-color textbook turns the learning process into a real pleasure, not only for students in natural products chemistry but also experienced professionals.