Найти книгу: "Gene Discovery for Disease Models"

This book provides readers with new paradigms on the mutation discovery in the post-genome era. The completion of human and other genome sequencing, along with other new technologies, such as mutation analysis and microarray, has dramatically accelerated the progress in positional cloning of genes from mutated models. In 2002, the Mouse Genome Sequencing Consortium stated that “The availability of an annotated mouse genome sequence now provides the most efficient tool yet in the gene hunter's toolkit. One can move directly from genetic mapping to identification of candidate genes, and the experimental process is reduced to PCR amplification and sequencing of exons and other conserved elements in the candidate interval. With this streamlined protocol, it is anticipated that many decades-old mouse mutants will be understood precisely at the DNA level in the near future.” The implication of such a statement should be similar to the identification of mutated genes from human diseases and animal models, when genome sequencing is completed for them. More than five years have passed, but genes in many human diseases and animal models have not yet been identified. In some cases, the identification of the mutated genes has been a bottleneck, because the genetic mechanism holds the key to understand the basis of the diseases. However, an integrative strategy, which is a combination of genetic mapping, genome resources, bioinformatics tools, and high throughput technologies, has been developed and tested. The classic paradigm of positional cloning has evolved with completely new concepts of genomic cloning and protocols. This book describes new concepts of gene discovery in the post-genome era and the use of streamlined protocols to identify genes of interest. This book helps identify not only large insertions/deletions but also single nucleotide mutations or polymorphisms that regulate quantitative trait loci (QTL).

Content

Actions in emergency situations

• Engine overheating actions
• Starting the engine from the battery of another car
• Fuse replacement
• Wheel replacement
• Towing a car

Daily checks and troubleshooting Instruction manual

• General information
• Locking and unlocking locks
• Seats and seat belts
• Instruments and controls

Precautions and safety rules when working on a car Basic tools, measuring instruments and methods of working with them

• Basic set of necessary tools
• Methods of working with measuring devices

Engine

• Technical specifications
• Petrol engines in volume of 1.3 l and 1.5 l (135)
• The petrol engine in volume of 1.5 l (4G1)
• The petrol engine in volume of 1.1 l (134)
• The diesel engine in volume of 1.5 l (639)
• Appendix to chapter

Power system

• Technical specifications
• Petrol engines in volume of 1.3 l and 1.5 l (135)
• The petrol engine in volume of 1.5 l (4G1)
• The diesel engine in volume of 1.5 l (639)
• Fuel supply system
• Appendix to chapter

Lubrication system

• Gasoline engines in volume of 1.3 l, 1.5 l (135) and 1.1 l (134)
• The petrol engine in volume of 1.5 l (4G1)
• The diesel engine in volume of 1.5 l (639)

Cooling system

• Technical specifications
• Basic work
• Thermostat
• Water pump
• Radiator

The intake and exhaust system

• Technical specifications
• Gasoline engines in volume of 1.3 l, 1.5 l (135) and 1.1 l (134)
• The petrol engine in volume of 1.5 l (4G1)
• The diesel engine in volume of 1.5 l (639)

Transmission

• Technical specifications
• Clutch
• Manual transmission
• Automatic transmission
• Appendix to chapter

Power shafts

• Technical specifications
• Removing and installing
• Disassembly and assembly
• Appendix to chapter

Chassis

• Technical specifications
• Front suspension
• Rear suspension
• Wheels and tires
• Appendix to chapter

Brake system

• Technical specifications
• Hydraulic drive
• Front brakes
• Rear brakes
• Parking brake
• Appendix to chapter

Steering

• Technical specifications
• Steering service
• Steering wheel and steering column
• Steering gear
• Appendix to chapter

Body

• Exterior
• Interior
• Doors
• Glasses
• Luke
• Body dimensions
• Appendix to chapter

Passive safety system

• Description
• Seat belts
• Airbags
• Disposing of system elements

Ventilation, air conditioning and heating system

• Technical specifications
• Heater and ventilation system
• Air conditioning (climate control)

Electrical equipment

• Starting system
• Charging system
• Ignition system (petrol)
• Preheating system (diesel)
• Headlamps
• Audio system

Electroschemes Explanatory dictionary

• Abbreviations

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Полный вариант заголовка: «An account of a successful method of treating diseases of the spine : With observations, and cases in illustration / By Thomas Baynton».

Полный вариант заголовка: «The history of the discovery and conquest of the Canary Islands : translated from a Spanish manuscript, lately found in the island of Palma : with an enquiry into the origin of the ancient inhabitants : to which is added, a dsescription of the Canary Islands, including the modern history of the inhabitants, and an account of their manners, customs, trade, & c. / by George Glas».