Скачать книгу - Biotransformations

This book addresses the use of ionic liquids in biotransformation and organocatalysis. Its major parts include: an overview of the fundamentals of ionic liquids and their interactions with proteins and enzymes; the use of ILs in biotransformations; non-solvent applications such as additives, membranes, substrate anchoring, and the use of ILs in organocatalysis (from solvents to co-catalysts and new reactivities, as well as non-solvent applications such as anchoring and immobilization).

Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities. Practical Methods for Biocatalysis and Biotransformations 2 is a «how-to» guide that focuses on the practical applications of enzymes and strains of microorganisms that are readily obtained or derived from culture collections. The sources of starting materials and reagents, hints, tips and safety advice (where appropriate) are given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology are given and relevant references to the primary literature are cited. This second volume – which can be used on its own or in combination with the first volume – concentrates on new applications and new enzyme families reported since the first volume. Contents include: introduction to recent developments and future needs in biocatalysts and synthetic biology in industry reductive amination enoate reductases for reduction of electron deficient alkenes industrial carbonyl reduction regio- and stereo- selective hydroxylation oxidation of alcohols selective oxidation industrial hydrolases and related enzymes transferases for alkylation, glycosylation and phosphorylation C-C bond formation and decarboxylation halogenation/dehalogenation/heteroatom oxidation tandem and sequential multi-enzymatic syntheses Practical Methods for Biocatalysis and Biotransformations 2 is an essential collection of biocatalytic methods for chemical synthesis which will find a place on the bookshelves of synthetic organic chemists, pharmaceutical chemists, and process R&D chemists in industry and academia.

Industrial Biotransformations – a user-friendly and application-oriented up-to-date overview of one-step biotransformations of industrial importance. The data conferring each process is arranged in a convenient format to survey so that the processes can easily be compared. Each set of data is accompanied by key literature citations. As far as flow sheets of the processes are available, these are given reduced to their significant elements. An extensive index classified by substrates, products, enzymes, and companies provides direct access to each process organized in the order of enzyme classes. The reader will find all significant parameters characterizing the biotransformation itself and the process.

The completely revised second edition of this user-friendly and application-oriented overview of one-step biotransformations of industrial importance. Based on extensive literature and patent research, this book is unique in arranging each process in a systematic way to allow for easy comparison. All the chapters have been rewritten, with all the processes updated and more than 30 new processes added. Each set of data is accompanied by key literature citations, supported by flow sheets where available, reduced to their significant elements. In addition, an extensive index classified by substrates, products, enzymes, and companies provides direct access to each process, organized according to enzyme class. Biotechnologists, biochemists, microbiologists, process engineers and those working in the chemical and biotechnological industries will find here all the significant parameters characterizing both the biotransformation and the process.

The development of new asymmetric catalytic methods is of fundamental importance to industrial synthetic chemistry. The demand for optically pure synthetic intermediates and the drive to adopt greener methods of synthesis have stimulated a growing interest in biocatalysis as a selective and environmentally benign synthetic technique. Practical Biotransformations: A Beginner's Guide provides an introduction to microbes and enzymes and demonstrates their practical applications in synthetic organic chemistry. Designed as a laboratory manual, this user-friendly guide discusses standard laboratory techniques, with appropriate advice on aspects of microbial practice and associated safety. Topics covered include: An introduction to equipment in a biotransformations laboratory An overview of biocatalyst sources Maintenance and growth of biocatalysts Example biotransformations using commercially available microbes and enzymes Basic gene cloning and the use of ‘designer’ biocatalysts This book will be a valuable resource for synthetic organic chemists with little or no experience of biochemistry or microbiology. It is the author’s hope that this text will inspire readers to consider biocatalytic methods as real alternatives to traditional synthetic solutions.