Скачать книгу - Evolutionary Methods in Biotechnology
Miniturization and high throughput assay technology have brought the power of molecular evolution to the bioscience laboratory. Applied wisely, the evolutionary approach can quickly yield the desired result even where other methods have failed. From library generation by random or directed mutagenesis to screening and selection techniques – the crucial steps for successful evolutionary biotechnology are described in detail in this practical guide that also includes valuable troubleshooting hints on frequently encountered problems. Modern methods for the surface display of peptides and proteins, selective enrichment of nucleic acid aptamers and high-throughput screening of industrial biocatalysts are explained, and computer-based methods for in silico protein and RNA engineering are described as an alternative to in vitro approaches. A special section covers the patenting regulations with regard to biotechnological innovations derived from directed evolution. As an added bonus, a CD-ROM is included that contains software tools for library design, selection of mutagenesis positions, and various predictive algorithms. In short, this practice oriented handbook is an indispensable tool for every scientist working in this interdisciplinary research area.


Repair of car interiors, methods, equipment, materials, in eBook Repair of car interiors, methods, equipment, materials, in eBook

Автор: Монолит

Год издания: 

Content

Introduction

Upholstery tools and materials

Manual dismantling of interior parts

Mastering the art of sewing

Making upholstery pattern

Examples of ready patterns

Covers

Installation instructions autocovers

Leather interior with their own hands

Padding of the saloon ceiling

We produce door skin

Padding vinyl interior

Padding upholstery motorcycle seats

Renovation of the old sagging armchair

Eliminating failures heated seat

Nano Textile upholstery

Technology smart car flocking

The interiors of the converted cars



Second Language Research Methods Second Language Research Methods

Автор: Herbert W. Seliger

Год издания: 

Based on a set of four research parameters, this book discusses the development of research questions and hypotheses, naturalistic and experimental research, data collection, and validation of research instruments. Each chapter includes examples and activities.


The Methods and Scope of Genetics The Methods and Scope of Genetics

Автор: Bateson William

Год издания: 



Methods of Authors Methods of Authors

Автор: Erichsen Hugo

Год издания: 



They Ask You Answer. A Revolutionary Approach to Inbound Sales, Content Marketing, and Today's Digital Consumer They Ask You Answer. A Revolutionary Approach to Inbound Sales, Content Marketing, and Today's Digital Consumer

Автор: Marcus Sheridan

Год издания: 

A revolutionary marketing strategy proven to drive sales and growth They Ask You Answer is a straightforward guide to fixing your current marketing strategy. Regardless of your budget, you are almost certainly overspending on television, radio, and print ads, yet neglecting the number-one resource you have at your disposal: the Internet. Content marketing is no longer about keyword-stuffing and link-building; in fact, using those tactics today gets your page shuffled to the bottom of the heap. Quality content is the key to success, and you already have the ingredients in-house. This book shows you how to structure an effective content strategy using the same proven principles that have revolutionized marketing for all types of businesses, across industries. Author Marcus Sheridan's pool company struggled after the housing collapse; today, they're one of the largest pool installers in the U.S., turning away millions of dollars in business they simply cannot accommodate every year. How did he manage it? He answered questions. This book shows you how Marcus's strategy can work for your business, and how to use your keyboard to bring customers through the door. Boost your company's web presence with methods that work Build a level of trust that generates customer evangelism Leverage your in-house resources to produce winning content Utilize tactics that work, regardless of industry or sector When people have questions, they ask a search engine. If you have answers, the right content strategy will get them to the top of the search results and seen by millions of eyes every day. Drop the marketing-speak, stop «selling,» and start answering. Be seen as an authority, not just another advertisement. They Ask You Answer describes a fresh approach to marketing and the beginning of big things for your business.