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A consolidated and comprehensive reference on ligand-binding assays Ligand-binding assays (LBAs) stand as the cornerstone of support for definition of the pharmaco-kinetics and toxicokinetics of macromolecules, an area of burgeoning interest in the pharmaceutical industry. Yet, outside of the Crystal City Conference proceedings, little guidance has been available for LBA validation, particularly for assays used to support macromolecule drug development. Ligand-Binding Assays: Development, Validation, and Implementation in the Drug Development Arena answers that growing need, serving as a reference text discussing critical aspects of the development, validation, and implementation of ligand-binding assays in the drug development field. Ligand-Binding Assays covers essential topics related to ligand-binding assays, from pharmacokinetic studies, the development of LBAs, assay validation, statistical LBA aspects, and regulatory aspects, to software for LBAs and robotics and other emerging methodologies for LBAs. Highlights include: A general discussion of challenges and proven approaches in the development of ligand-binding assays More detailed examination of characteristics of these assays when applied to support of pharmacokinetic and toxicokinetic studies of compounds at different stages in the discovery or development timeline A concise, but detailed, discussion of validation of ligand-binding assays for macromolecules A practical approach to «fit-for-purpose» validation of assays for biomarkers, those molecules receiving increased attention as potentially demonstrating that the target chosen in discovery is being modulated by the candidate therapeutic, both in nonclinical and clinical studies Written by a team of world-recognized authorities in the field, Ligand-Binding Assays provides key information to a broad range of practitioners, both in the pharmaceutical and allied industries and in related contract research organizations and academic laboratories and, perhaps, even in the field of diagnostics and clinical chemistry.

Calcium Binding Proteins explains the unique and highly diverse functions of calcium in biology, which are realized by calcium binding proteins. The structures and physical characteristics of these calcium binding proteins are described, as well as their functions and general patterns of their evolution. Techniques that underlie the description of proteins are discussed, including NMR, circular dichroism, optical rotatory dispersion spectroscopy, calorimetry,and crystallography. The book discusses the patterns of bochmical phenomena such as calcium homeostasis, mineralization, and cell signaling that involve specific proteins. It summarizes ongoing research and presents general hypotheses that help to focus future research, and also provides a conceptual framework and a description of the underlying techniques that permits someone entering the field to become conversant.

Presents the physical background of ligand binding and instructs on how experiments should be designed and analyzed Reversible Ligand Binding: Theory and Experiment discusses the physical background of protein-ligand interactions—providing a comprehensive view of the various biochemical considerations that govern reversible, as well as irreversible, ligand binding. Special consideration is devoted to enzymology, a field usually treated separately from ligand binding, but actually governed by identical thermodynamic relationships. Attention is given to the design of the experiment, which aids in showing clear evidence of biochemical features that may otherwise escape notice. Classical experiments are reviewed in order to further highlight the importance of the design of the experiment. Overall, the book supplies students with the understanding that is necessary for interpreting ligand binding experiments, formulating plausible reaction schemes, and analyzing the data according to the chosen model(s). Topics covered include: theory of ligand binding to monomeric proteins; practical considerations and commonly encountered problems; oligomeric proteins with multiple binding sites; ligand binding kinetics; hemoglobin and its ligands; single-substrate enzymes and their inhibitors; two-substrate enzymes and their inhibitors; and rapid kinetic methods for studying enzyme reactions. Bridges theory of ligand binding and allostery with experiments Applies historical and physical insight to provide a clear understanding of ligand binding Written by a renowned author with long-standing research and teaching expertise in the area of ligand binding and allostery Based on FEBS Advanced Course lectures on the topic Reversible Ligand Binding: Theory and Experiment is an ideal text reference for students and scientists involved in biophysical chemistry, physical biochemistry, biophysics, molecular biology, protein engineering, drug design, pharmacology, physiology, biotechnology, and bioengineering.

The most captivating novel of 2019‘Utterly brilliant’ Joanna Cannon ‘Truly spellbinding’ Guardian ‘Pure magic’ Erin Kelly ‘A real treat’ The Times ‘Gorgeous' Stella Duffy ‘Astounding’ Anna MazzolaImagine you could erase your grief. Imagine you could forget your pain. Imagine you could hide a secret. Forever.Emmett Farmer is working in the fields when a letter arrives summoning him to begin an apprenticeship. He will work for a Bookbinder, a vocation that arouses fear, superstition and prejudice – but one neither he nor his parents can afford to refuse.He will learn to hand-craft beautiful volumes, and within each he will capture something unique and extraordinary: a memory. If there’s something you want to forget, he can help. If there’s something you need to erase, he can assist. Your past will be stored safely in a book and you will never remember your secret, however terrible.In a vault under his mentor’s workshop, row upon row of books – and memories – are meticulously stored and recorded.Then one day Emmett makes an astonishing discovery: one of them has his name on it.THE BINDING is an unforgettable, magical novel: a boundary-defying love story and a unique literary event.

An essential tool for all reading groups – a detailed guide to exciting debut novel, The Binding!A comprehensive guide to Bridget Collins’ The Binding, this discussion aid includes a wealth of information and resources: useful literary context; an author biography; a plot synopsis; analyses of themes and imagery; character analysis; thought-provoking discussion questions; recommended further reading, an exclusive Q&A with the author and even a quick quiz.This companion guide takes the hard work out of preparing for meetings and guarantees productive discussion. For solo readers, it encourages a deeper examination of a rich and rewarding text.